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Ebrahimi, F., Bakhshi, M., Honari, H., Hajizadeh, A. (2011). Recombinant Expression of Human Epidermal Growth Factor in E. coli and Assessment of its Effect in NIH-3T3 Cell line. Electronic and Cyber Defense, 2(4), 325-332.
Firouz Ebrahimi; Mostafa Bakhshi; Hosain Honari; Abbas Hajizadeh. "Recombinant Expression of Human Epidermal Growth Factor in E. coli and Assessment of its Effect in NIH-3T3 Cell line". Electronic and Cyber Defense, 2, 4, 2011, 325-332.
Ebrahimi, F., Bakhshi, M., Honari, H., Hajizadeh, A. (2011). 'Recombinant Expression of Human Epidermal Growth Factor in E. coli and Assessment of its Effect in NIH-3T3 Cell line', Electronic and Cyber Defense, 2(4), pp. 325-332.
Ebrahimi, F., Bakhshi, M., Honari, H., Hajizadeh, A. Recombinant Expression of Human Epidermal Growth Factor in E. coli and Assessment of its Effect in NIH-3T3 Cell line. Electronic and Cyber Defense, 2011; 2(4): 325-332.

Recombinant Expression of Human Epidermal Growth Factor in E. coli and Assessment of its Effect in NIH-3T3 Cell line

علوم و فناوریهای پدافند نوین
Article 9, Volume 2, Issue 4 - Serial Number 6, May 2011, Pages 325-332    PDF (473.2 K)
Authors
Firouz Ebrahimi* ; Mostafa Bakhshi; Hosain Honari; Abbas Hajizadeh
Receive Date: 30 January 2019,  Revise Date: 16 July 2025,  Accept Date: 30 January 2019 
Abstract
The EGF family include13 members and the hEGF as founding member of this family involves in epidermal wound healing. Epidermal growth factor is an essential polypeptide for wound healing and promoting mitotic division. In this study, human epidermal growth factor gene was adapted from gene bank and its codons were optimized according to codon preference of E. coli BL21. The gene was prepared as synthetic construct in pET21a (+). Then the recombinant construct was transferred to E. coli and hEGF gene in the vector was expressed under the control of inducible T7 promoter. Purification was done using affinity chromatography. The bioassay results of three different doses showed that recombinant protein has significant effects on growth of NIH-3T3 cell line.
Keywords
recombinant hEGF; Synthetic gene; E. coli; NIH-3T3 cell line
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