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Najafi, M., Musavi, S., Nezamoleslam, T. (2019). Electrochemical Nanobiosensor Based on Immobilization of Acetylcholinesterase to Multiwall Carbon Nanotube Modified Glassy Carbon Electrode for Determination of Triethylthiophosphate. Electronic and Cyber Defense, 4(4), 271-277.
M. Najafi; S. H. Musavi; T. Nezamoleslam. "Electrochemical Nanobiosensor Based on Immobilization of Acetylcholinesterase to Multiwall Carbon Nanotube Modified Glassy Carbon Electrode for Determination of Triethylthiophosphate". Electronic and Cyber Defense, 4, 4, 2019, 271-277.
Najafi, M., Musavi, S., Nezamoleslam, T. (2019). 'Electrochemical Nanobiosensor Based on Immobilization of Acetylcholinesterase to Multiwall Carbon Nanotube Modified Glassy Carbon Electrode for Determination of Triethylthiophosphate', Electronic and Cyber Defense, 4(4), pp. 271-277.
Najafi, M., Musavi, S., Nezamoleslam, T. Electrochemical Nanobiosensor Based on Immobilization of Acetylcholinesterase to Multiwall Carbon Nanotube Modified Glassy Carbon Electrode for Determination of Triethylthiophosphate. Electronic and Cyber Defense, 2019; 4(4): 271-277.

Electrochemical Nanobiosensor Based on Immobilization of Acetylcholinesterase to Multiwall Carbon Nanotube Modified Glassy Carbon Electrode for Determination of Triethylthiophosphate

علوم و فناوریهای پدافند نوین
Article 5, Volume 4, Issue 4 - Serial Number 14, February 2014, Pages 271-277    PDF (529.3 K)
Authors
M. Najafi* ; S. H. Musavi; T. Nezamoleslam
Receive Date: 30 January 2019,  Revise Date: 16 July 2025,  Accept Date: 30 January 2019 
Abstract
A simple method has been developed for immobilization of acetylcholinesterase (AChE) covalent bonding to a multiwall carbon nanotube (MWNT) modified glassy carbon electrode and a sensitive biosensor for rapid determination of triethylethiophosphate (nerve agent (VX) simulant) is proposed. MWNT improved the interface enzymatic hydrolysis reaction and increased the voltammetric response of the sensor. Under optimum conditions, the inhibition of triethylethiophosphate on AChE increased linearly to triethylethiophosphate in the 0.13 to 0.44 nM concentration range. The detection limit was 0.093 nM taken as the concentration equivalent to 10% of inhibition. The reproducibility of the biosensors obtained under optimum condition was good: RSD of 3% was observed for five replicates using the same biosensor. The sensor was found to be stable for over 2 months.
Keywords
Nanobiosensor; Acetylcholinesterase; Carbon nanotube; Triethylthiophosphate; Nerve Agent
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